Thymocyte Transfer to Enucleated Oocytes in the Mouse.
نویسندگان
چکیده
منابع مشابه
Somatic and embryonic cell nucleus transfer into intact and enucleated immature mouse oocytes.
BACKGROUND The aim of our study was to evaluate the possibility of embryonic or somatic cell haploidization after fusion with intact or enucleated immature oocytes which were subsequently cultured in vitro. Embryonic or somatic cell nuclei do not undergo premature chromosome condensation when fused to intact or enucleated immature oocytes whose maturation is prevented by dibutyryl cyclic AMP (d...
متن کاملEnucleated Oocytes and Zygotes
The present study was conducted to examine the development of nucLear transplant embryos produced by transplanting nucLei to either oocytes or zygotes in the mouse. Metaphase II oocytes and one-cell zygotes were enucleated and fused with transferred nuclei from late two-, fourand eight-cell stage embryos. Enucleation of metaphase oocytes was achieved using the interference microscope without st...
متن کاملDevelopmental ability of mouse late 2-cell stage blastomeres fused to chemically enucleated oocytes in vitro.
The effects of different concentrations of etoposide and cycloheximide (ETO-CHXM), used for chemical enucleation of mouse oocytes, on polar body extrusion and chromatin expulsion were tested. The developmental ability of blastomeres of late 2-cell stage embryos fused to chemically enucleated oocytes of different ages or cytoplasts from different sources was also examined in vitro. Metaphase I o...
متن کاملDevelopment of mouse embryonic nuclei transferred to enucleated oocytes and zygotes.
The present study was conducted to examine the development of nuclear transplant embryos produced by transplanting nuclei to either oocytes or zygotes in the mouse. Metaphase II oocytes and one-cell zygotes were enucleated and fused with transferred nuclei from late two-, four- and eight-cell stage embryos. Enucleation of metaphase oocytes was achieved using the interference microscope without ...
متن کاملDynamic Reorganization of Nucleosome Positioning in Somatic Cells after Transfer into Porcine Enucleated Oocytes
The nucleosome, the fundamental structural unit of chromatin, is a critical regulator of gene expression. The mechanisms governing changes to nucleosome occupancy and positioning during somatic cell reprogramming remain poorly understood. We established a method for generating genome-wide nucleosome maps of porcine embryonic fibroblasts (PEF), reconstructed 1-cell embryos generated by somatic c...
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ژورنال
عنوان ژورنال: Journal of Reproduction and Development
سال: 1993
ISSN: 0916-8818,1348-4400
DOI: 10.1262/jrd.39.301